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Tip: To quickly search the same gene in another data set, click the links from the last column. The locuslink number will be used as query.
|No||probe set||Symbol||gene||Accession||LocusLink||Description||GFP||FSK_GFP||(FSK_GFP vs GFP) FC||(FSK_GFP vs GFP) LFC||FSK_ACREB||(FSK_ACREB vs FSK_GFP) FC||(FSK_ACREB vs FSK_GFP) LFC||ACREB||(ACREB vs GFP) FC||(ACREB vs GFP) LFC||Link|
|1||160312_at||Cklfsf6||RIKEN cDNA 2810051A14 gene||AW125031||67213||"Cluster Incl AW125031:UI-M-BH2.1-apx-a-02-0-UI.s1 Mus musculus cDNA, 3 end /clone=UI-M-BH2.1-apx-a-02-0-UI /clone_end=3 /gb=AW125031 /gi=6100561 /ug=Mm.28858 /len=473 /NOTE=replacement for probe set(s) 94474_at on MG-U74A"||182.22||199.41||1.09||0.9||174.62||-1.14||-0.82||194.37||1.07||0.93||"all, "||Liver_Expression Mouse_CRE_Prediction|
Culture of mouse pancreatic beta cell line MIN6 cells were infected with GFP or ACREB adenovirus, and treated with cAMP agonist forskolin (FSK) for 1hr. RNA was extracted using the Qiagen RNeasy kit. Two controls and two forskolin treated samples from GFP or ACREB virus infected cells were analyzed.
Total RNA samples were labeled and hybridized by standard protocol to Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A array. Array data was analyzed using the dChip software with PM-MM models.
Guidelines for Choosing cAMP Induced Genes in this Data
The fold change (FC) value is positive for increased expression and negative for decrease expression. There is also a lower bound of fold change (LFC), which is a conservative estimation of the fold change with 90% confidence. For this data set, if a gene was induced by FSK in GFP infected samples (LFC>=1) and this induction was inhibited by ACREB ((FSK_ACREB vs FSK_GFP) LFC<=-1), we consider it as a good cAMP induced gene and therefore a potential CREB target.